Journal: Journal of leukocyte biology

Article Title: Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation

doi: 10.1002/JLB.3MA1017-423R

Figure Lengend Snippet: (A) Eosinophils in the jejunum of control and SPI-challenged mice with and without t-TUCB treatment assessed by immunohistology with rat mAb against MBP (stained dark brown). A representative image is shown for each group. (B) Quantitation of cells positive for expression of MBP in the villi of the above groups of mice. (C) Eotaxin-1 and 2 levels in jejunal tissue lysates of control and SPI-challenged mice with and without t-TUCB treatment by ELISA. (D) Dual immunostaining of jejunal sections from SPI-challenged mice with antibodies against sEH (green) and eosinophil-specific EPX (red). Arrows indicate sEH-positive/EPX-positive cells in the villi. Immunoreactivity in jejunal sections treated with control IgG is also shown. Data representative of 3 mice are shown. (E) Prevalence of mast cells in control and SPI-challenged mice with and without t-TUCB treatment based on CAE staining (stained dark purple). Arrows indicate CAE-positive cells. A representative image is shown for each group. (F) Quantitation of CAE-positive cells in the lower lamina propria of the above groups of mice. Scale bar, 100 μm in A and E, 20 μm in C. Combined data (mean ± SEM) of n = 9–12 mice/group in B and F and 6–8 mice/group in D are shown. **p<0.01 in B and *p< 0.05 in F versus SPI.

Article Snippet: Differentiated cells were evaluated for expression of MBP by confocal microscopy using rat mAb against murine MBP (2.5 μg/ml) followed by FITC-conjugated goat anti-rat IgG and of Siglec-F by flow cytometry (FACScan and CellQuest Pro ™ Software, BD Biosciences, San Diego, CA) with PE-conjugated rat anti-mouse Siglec-F (5 μg/ml, BD Biosciences).

Techniques: Staining, Quantitation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Immunostaining

Journal:

Article Title: Critical Role for Protein Tyrosine Phosphatase SHP-1 in Controlling Infection of Central Nervous System Glia and Demyelination by Theiler's Murine Encephalomyelitis Virus

doi: 10.1128/JVI.76.16.8335-8346.2002

Figure Lengend Snippet: Focal demyelinating lesions in dorsal cervical spinal cords of moth-eaten (me/me) mice at 5 days after i.c. inoculation with the attenuated BeAn 8386 strain of TMEV. Five-micrometer paraffin sections were stained with rat monoclonal antibody to MBP and labeled with FITC-conjugated secondary antibody. Sections were photographed with color film by double exposure under both FITC (green) and red fluorescence filter sets. Green fluorescence labels MBP. Red profiles above the background in panel D indicate autofluorescent red blood cells in focal hemorrhagic lesions in regions of demyelination in the parenchyma of the spinal cord. The dorsal funiculus of the cervical spinal cord is shown in either TMEV-infected (B and D) or sham-infected (A and C) normal littermate mice (A and B) and me/me mice (C and D).

Article Snippet: The spinal cord sections were incubated with monoclonal antibodies to either MBP (rat monoclonal immunoglobulin G [IgG] against MBP; MCA 409; Accurate Chemical and Scientific Corp., Westbury, N.Y.), PLP (mouse monoclonal IgG against myelin PLP; clone plpc1; Oncogene Research Products, Cambridge, Mass.), or GFAP (rat monoclonal IgG; Zymed Laboratories, South San Francisco, Calif.) overnight; this step was followed by rinsing and incubating the sections in goat anti-rat or anti-mouse IgG conjugated to tetramethyl rhodamine isothiocyanate (TRITC; Zymed).

Techniques: Staining, Labeling, Fluorescence, Infection

Journal:

Article Title: Critical Role for Protein Tyrosine Phosphatase SHP-1 in Controlling Infection of Central Nervous System Glia and Demyelination by Theiler's Murine Encephalomyelitis Virus

doi: 10.1128/JVI.76.16.8335-8346.2002

Figure Lengend Snippet: Double immunofluorescence of MBP (TRITC; red) and TMEV (FITC; green) in the brain 5 days after inoculation with TMEV. Five-micrometer paraffin sections were stained with rat monoclonal antibody to MBP and labeled with TRITC-conjugated secondary antibody. TMEV antibodies were detected with FITC-conjugated antibodies. Sections were photographed with color film by double exposure of the same frame under both FITC and TRITC filter sets. (A) ×400 magnification of the corpus callosum (lower) at the interface with the cerebral cortex (upper). (B) Higher magnification (×630) of an area similar to that in panel A but centered at the interface, where myelination is sparse, to allow resolution of doubly labeled cells (brownish yellow cell bodies indicated by arrows).

Article Snippet: The spinal cord sections were incubated with monoclonal antibodies to either MBP (rat monoclonal immunoglobulin G [IgG] against MBP; MCA 409; Accurate Chemical and Scientific Corp., Westbury, N.Y.), PLP (mouse monoclonal IgG against myelin PLP; clone plpc1; Oncogene Research Products, Cambridge, Mass.), or GFAP (rat monoclonal IgG; Zymed Laboratories, South San Francisco, Calif.) overnight; this step was followed by rinsing and incubating the sections in goat anti-rat or anti-mouse IgG conjugated to tetramethyl rhodamine isothiocyanate (TRITC; Zymed).

Techniques: Immunofluorescence, Staining, Labeling